Genetic Predisposition to Chronic Dust Bronchitis among Potash Miners

 

L.V. Dubovskaya, T.M. Rybina, Y.S. Bakakina, O.F. Kardash, N.P. Denisevich and I.D. Volotovski

 

Research Paper I Published August,2015

 

Journal of Medical and Biological Science Research Vol. 1 (6), pp. 55-61

 

ABSTRACT

Chronic respiratory diseases represent a prevalent occupational disease worldwide. It is suggested that polymorphisms in genes of enzymes of monooxygenase (microsomal epoxide hydrolase 1 (EPHX1), cytochromes (CYPs)) and antioxidant glutathione S-transferases (GSTs) systems might potentiate susceptibility to respiratory system pathology in workers employed in mining. There are a large number of studies on genetic markers for occupational pulmonary diseases, but the majority of them concerns workers of the coal industry. Therefore, we aimed to investigate genetic predisposition to chronic dust occupational bronchitis (СВ) among potash miners. The study population consisted of 38 males with CB and 18 controls. Deletions in GSTM1 and GSTT1 genes were analysed using multiplex PCR. Polymorphisms of CYP1А1 (Ile462Val), EPHX1 (His139Arg) and GSTP1 (Ile105Val) were detected using PCR followed by restriction fragment length polymorphism (PCR-RFLP) analysis. Significantly increased risk for CB was observed for subjects with polymorphism in exon5 (Ile105Val) of GSTP1 (ОR=8.6, χ²=8.794, р=0.001) and polymorphism in exon4 (His139Arg) of EPHX1 (ОR=8.6, χ²=4.4, р=0.04). This study showed that the GSTP1 (Ile105Val) and EPHX1 (His139Arg) polymorphisms are closely associated with the increased risk for chronic dust occupational bronchitis among potash miners.

Key words: Bronchitis, Genetic polymorphism, Potash miners, Predisposition.

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