Journal of Agricultural Science and Food Technology

Research Paper|Published April 2020|Vol.6 (4):69-78

https://doi.org/10.36630/jasft_20009

 

 

Isolation of Collagen from Sailfin Sandfish Skins as By-product for Use in Some Industrial Purposes

 

Takeshi Nagai^1,2,3*, Masataka Saito⁴, Yasuhiro Tanoue⁵, Norihisa Kai⁶ and Nobutaka Suzuki⁷

 

¹Graduate School of Agricultural Sciences, Yamagata University, Yamagata 9978555, Japan.
²The United Graduate School of Agricultural Sciences, Iwate University, Iwate 0208550, Japan.
³Graduate School, Prince of Songkla University, Songkhla 90112, Thailand. ⁴Kagawa Nutrition University, Saitama 3500288, Japan.
⁵National Fisheries University, Yamaguchi 7596595, Japan.
⁶Oita University, Oita 8701192, Japan.
⁷Nagoya Research Institute, Aichi 4701131, Japan.

 

ABSTRACT

The objective of this study was to isolate and characterize collagen from sailfin sandfish skins as an useful resource for potential in commercial applications. The fats and pigments of the skins could easily be removed by cold acetone treatment. High-purity, pure-white, and odorless collagen, with a chain composition of α1α2α3, was successfully obtained with high-yield approximately 31.0 % on dry skin’s weight basis. Denaturation temperature was relatively high, approximately 30 °C as well as that of bovine Achilles tendon collagen, although sailfin sandfish lived in the Japan Sea where the water temperature was low. ATR-FTIR analysis showed that the secondary structure was different from that of bovine Achilles tendon collagen. The finding suggests that collagen from sailfin sandfish waste skins may be applied not only to food products and nutraceuticals as materials of edible sausage casings and gelatin but also to cosmetics, pharmaceuticals, and advanced biomedical applications as peptides.

 

Keywords: Marine collagen, Acetone treatment, Characterization, Effective utilization, High-yield, Waste material.

 

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