Article Abstract

Journal of Agricultural Science and Food Technology

Research Paper|Published April 2020|Vol.6 (4):69-78



Isolation of Collagen from Sailfin Sandfish Skins as By-product for Use in Some Industrial Purposes


Takeshi Nagai1,2,3*, Masataka Saito4, Yasuhiro Tanoue5, Norihisa Kai6 and Nobutaka Suzuki7


1Graduate School of Agricultural Sciences, Yamagata University, Yamagata 9978555, Japan.
2The United Graduate School of Agricultural Sciences, Iwate University, Iwate 0208550, Japan.
3Graduate School, Prince of Songkla University, Songkhla 90112, Thailand. 4Kagawa Nutrition University, Saitama 3500288, Japan.
5National Fisheries University, Yamaguchi 7596595, Japan.
6Oita University, Oita 8701192, Japan.
7Nagoya Research Institute, Aichi 4701131, Japan.



The objective of this study was to isolate and characterize collagen from sailfin sandfish skins as an useful resource for potential in commercial applications. The fats and pigments of the skins could easily be removed by cold acetone treatment. High-purity, pure-white, and odorless collagen, with a chain composition of α1α2α3, was successfully obtained with high-yield approximately 31.0 % on dry skin’s weight basis. Denaturation temperature was relatively high, approximately 30 °C as well as that of bovine Achilles tendon collagen, although sailfin sandfish lived in the Japan Sea where the water temperature was low. ATR-FTIR analysis showed that the secondary structure was different from that of bovine Achilles tendon collagen. The finding suggests that collagen from sailfin sandfish waste skins may be applied not only to food products and nutraceuticals as materials of edible sausage casings and gelatin but also to cosmetics, pharmaceuticals, and advanced biomedical applications as peptides.


Keywords: Marine collagen, Acetone treatment, Characterization, Effective utilization, High-yield, Waste material.


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