Biochemical Effect of Ethanolic Extract of Phyllantus Amarus (L.) On Gentamicin-Induced Liver and Kidney Damage in Rats

 

Sule O.J. and Arhoghro M.E
 

 

Full Length Research Paper I Published July, 2016

 

Journal of Medical and Biological Science Research Vol. 2 (7), pp. 114-117

 

ABSTRACT

Hepato protective and nepro protective activity of ethanolic extract of Phyllanthus amarus leaves was assessed. Twenty five wistar albino rats were used, comprising of five groups of five animals each and were fed with growers’ match and water freely. Rats in groups B and C were administered 200 and 300 mg/kg, respectively, of ethanol extract of P. amarus orally for twenty one days. Groups A and E received normal saline water for the same period, while group C received 100 mg/kg of vitamin C. On the 21^st day the of study groups (B, C, D and E) were administered single dose of 80 mg/kg of gentamicin intraperitoneally, fasted for 24 h, then sacrificed on the 22^nd day and blood samples were collected through cardiac puncture. Samples were used to determine total protein, urea, total bilirubin and direct bilirubin concentrations using standard kits. The results showed a significant increase p<0.05 in the level of total protein in rat group A (normal control) compared to group E (positive control). Also, the level of total protein increased significantly in rats groups B, C and D pretreated with extracts and vitamin C, respectively, compared to group E. However, there was significant decrease p<0.05 in the levels of urea, total bilirubin and direct bilirubin in extract pretreated rats groups (B and C), compared to untreated rats group E. Conclusively, the findings indicated that the administration of ethanol extract of P. amarus at these concentrations confers hepato protective and nephron protective effects on gentamicin-induced rats.

Key words: Hepato protective, Nephro protective, Hepato protective and P. amarus.

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