Analysis of Cytokine Production in Household Contacts of Leprosy
Lorena Bruna P. de Oliveira, Pedro Henrique F. Marçal,
Rafael Silva Gama, Alexandre Castelo Branco, Regina L.
B. Cipriano, Erica Barbosa Magueta, Márcio Luís Moreira
de Souza, Euzenir N. Sarno and Lucia Alves de Oliveira
Fraga
Full Length Research Paper I Published February, 2016
Journal of Medical and Biological Science Research Vol.
2 (2), pp. 14-26
ABSTRACT
Analyze cytokine production in household contacts of
leprosy, paucibacillary (CPB) and multibacillary (CMB) after
one year of follow-up. Eighty-three individuals attended the
second evaluation. Peripheral blood mononuclear cells (PBMC)
of the subjects were stimulated with Mycobacteriu leprae,
PHA and cytokines were assessed by ELISA and Cytometry.
The results showed significant correlation between TNF-α and
age of the CMB in unstimulated PBMC culture. Grouping these
individuals is ≤ 20 and ≥ 21 years it was observed that ≥ 21
years had higher levels of TNF-α. It was found higher levels
of IL-10 in female CPB ≤ 20 years in cultures stimulated
with M. leprae. CMB showed a higher frequency of
TCD8+IL-10+ cells stimulated in culture with M. leprae
when compared with CPB.
It was found
that CMB whose index case had a treatment time ≤ 2 years,
showed a higher frequency of TCD8+IL-10+ cells stimulated in
culture with M. leprae when compared with CPB. It was
found that female’s ≥ 21 years of CMB showed a higher
frequency of TCD4+IL-4+ cells in culture without stimulation
than female ≤ 20 years. In conclusion, CMB displays a
modulatory response with increased IL-10; in contrast, CPB
shows a pro-inflammatory response with high levels of IFN-γ
and TNF- α.
Key words: Leprosy, Household contacts and Cytokines.
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