Protective Potentials of Bryophyllum pinnatum In Wistar Albino Rats Against Gentamicin-Induced Biochemical Injury

 

Sule, O.J* and Arhoghro, M.E
 

 

Full Length Research Paper I Published December, 2016

 

Journal of Medical and Biological Science Research Vol. 2 (10), pp. 163-167

 

ABSTRACT

The protective potentials of ethanol leaf extract of Bryophyllum pinnatum against gentamicin induced biochemical injury in Wistar albino rats were evaluated using serum biochemical parameters. Twenty-five male albino rats were divided randomly into five groups A to E. Groups C and D received 150 and 300 mg/kg B. pinnatum, respectively. Groups A and B were fed with chow (normal and positive controls) while Group E received 100 mg/kg of Vitamin C. Biochemical injury was induced in rats in groups B-E with gentamicin (i.p) at the dose of 80 mg/kg body weight on the 16th day of study. The rats were then fasted for 48 h and sacrificed by cervical dislocation. Serum was collected for biochemical analysis using standard methods and analytical biochemical kits. There was a significant increase in the serum levels of AST, ALT and ALP of the rats administered with gentamicin (Group B - positive control) compared to the Group A- normal control. However, treatment of rats with 150 and 300 mg/kg body weight of ethanol leaf extract of B. pinnatum significantly decrease these biochemical parameters compared to Group B (p < 0.05). Also, there was a significant increase in the serum levels of conjugated bilirubin and total bilirubin in Group B compared to the normal control. Rat pretreated with 150 and 300 mg/kg body weight of extract showed significant decrease in the conjugated and total bilirubin compared to the non-treated rats in Group B (p <0.05). Serum level of urea and creatinine significantly increased in Group B compared to normal control. Rats in groups C and D pretreated with 150 and 300 mg/kg body weight of extract showed significant decrease in the levels of urea and creatinine compared to Group B (p <0.05). There was significant decrease in the serum levels of albumin and total protein of the rats administered with gentamicin compared to the normal control. Rats pretreated with 150 and 300 mg/kg body weight of extract showed significant increase compared rats in non-treated Group B (p <0.05). The results obtained from this study shows that the ethanolic leaf extract of B. pinnatum has protective functions against gentamicin-induced hepatic and nephrotic damage in Wistar albino rats.

Key words: Bryophyllum pinnatum, Gentamicin-induced injury, Conjugated bilirubin, Total protein and Creatinine.

Full Text-PDF