Evaluation of the Gonadoprotective Effects of Allanblackia floribunda Oliver (Clusiacea) On Testes and Accessory Organs of Wistar Rats
Olasantan O.D, Areola J.O, Ayannuga O.A and Babalola O.O
Research Paper I Published November,2015
Journal of Medical and Biological Science Research Vol.
1 (9), pp. 134-144
ABSTRACT
This study investigated the
effect of the crude ethanolic extract and fractions of the
stem bark of Allanblackia floribunda
on the testes and accessory organs of rats. Forty five (45)
male rats of average weight between 150 to 200 g were
distributed into nine groups of five rats per group. Rats in
group 1 (control group) were administered distilled water,
while groups 2 to 9 were orally administered 200 and 300
mg/kg body weight of the
crude ethanolic extracts and aqueous, ethyl acetate and
butanol
fractions, once daily for 28 days. On
the 29th day, the animals were sacrificed;
weights of the testes, epididymis, seminal vesicle and
prostate glands were taken and documented.
Testicular antioxidant level was
determined by measuring testicular catalase and glutathione
peroxidise
activity.
Serum alkaline phosphatase, acid phosphatase, lactate
dehydrogenase and glucose-6-phosphate dehydrogenase
activities were also assayed for. Statistical analysis to
test significant difference between mean values was
performed using One-way Analysis of Variance (ANOVA).The
results revealed that crude ethanolic extract of
A. floribunda
gave positive reactions for the presence of alkaloids,
flavonoids, saponins, tanins, triterpenes and cardiac
glycosides and the median lethal dose (LD50) was
higher than 5000 mg/kg body weight. The acid phosphatase (ACP)
across all the groups showed an increase in activity when
compared with control. However, a general decrease in
alkaline phosphatase (glucose-6-phosphate dehydrogenase and
catalase activities was observed in all the groups with the
mean values of Group 2, Group 3 and Group 9 ACP; Group 6 and
7ALP; Group 2LDH and Groups 5, 6, 7, 8 and 9 G6PDH having a
significant difference (p<0.05) when compared with control.
Glutathione peroxidase activity (GPx) varied across all
groups with most groups having slightly lower activity (0.19+0.01
U/L/mg protein, 0.15+0.01 U/L/mg protein, 0.18+0.01
U/L/mg protein, 0.17+0.01 U/L/mg protein, 0.16+0.01
U/L/mg protein, 0.15+0.01 U/L/mg protein) when
compared with the control group (0.21+0.02 U/L/mg
protein). With the exception of Group 7 (300 mg/kg ethyl
acetate), all groups had slightly lower seminal vesicle,
testicular and epididymis weights when compared with the
control. The study concluded that the ethanolic extract and
fractions of A. floribunda had deleterious effects on
the testes and accessory organs of the animals, most
especially at 300 mg/kg dosage of the crude ethanolic
extract.
Key words: Allanblackia floribunda, Accessory
organs, Testis, Ethanolic Extract.
- Journal of Agricultural Science and Food Technology (JASFT)
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