Assessment of acute toxicity and LD₅₀ of Moringa oleifera ethanolic leave extract in albino rats and rabbits

 

Osman HM¹*, Shayoub ME², Babiker EM³, Faiza AO⁴, Munzir ME Ahmed⁵, Bashier Osman⁶, Ali Mohamed Elhassan⁷ and Taha KK⁸

 

Research Paper I Published June,2015

 

Journal of Medical and Biological Science Research Vol. 1 (4), pp. 38-43

 

¹Department of Biochemistry, Faculty of Pharmacy, University of National Ribat, Khartoum, Sudan.
²Department of Pharmaceutics, Faculty of Pharmacy, University of Khartoum, Sudan.
³Department of Zoology, Faculty of Sciences, University of Khartoum, Sudan.
⁴Veterinary Research Institute, Ministry of Animals’ Foundation, Sudan
⁵Department of Biochemistry, Faculty of Medicine, University of Gadarf, Khartoum, Sudan.
⁶Department of Pharmacology, Faculty of Pharmacy, University of Khartoum, Sudan.
⁷Faculty of Pharmacy, University of Khartoum, Sudan.
⁸College of Applied & Industrial Sciences, University of Bahri, Khartoum, Sudan.
 

*Corresponding author E-mail:hisham1212ribat@yahoo.com. Tel. +249912966220.     

 

Abstract

Leaves of Moringa oleifera extracted in 70% ethanol were assessed experimentally for acute toxicity in two groups each of six, one of albino rats and the other of rabbits. Each animal of the two groups was injected via intraperitoneal route with 150 mg/ml every five minutes till death occurred. The lethal dose of acute toxicity was found to be 6616.67mg/kg body weight (BW) for rats and 26043.67 mg/kg BW for rabbits. Injection with 14ml of concentrated form of 450mg/ml over 10 minutes showed no death among albino rats. The results of histopathology confirmed that the death of both animals was due to excessive fluid injected for the acute toxicity test. Based on these results it can be concluded that the plant had low toxicity effect when given in concentrated doses for short period of time.

Key word: Moringa oleifera, acute toxicity, LD₅₀, albino rats, rabbits, medicinal plant.

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