JMBSR-Abstract

Cadmium Status and Activities of Cardiac Marker Enzymes in Smokers: Using Experimental Animals Model

 

Agbaogun B.K.O and Babalola O.O.
 

 

Research Paper I Published December,2015

 

Journal of Medical and Biological Science Research Vol. 1 (10), pp. 179-182

 

ABSTRACT

This study was designed to determine blood cadmium concentration and the activities of selected cardiac marker enzymes in two different groups of smokers, using experimental animal model. Twelve wiener rabbits were randomly distributed into 3 groups of 4 animals each. Groups I and II animals were exposed to smoke from two different brands of cigarette commonly consume in Nigeria for a period of three weeks. Each animal in the test groups was treated with six sticks of cigarette per day in an inhalation chamber. Group III animals served as the controls were exposed to normal air. At the end of the test period, the animals were sacrificed. The blood samples were collected and blood Cadmium concentration (bCd) were determined by Atomic Absorption Spectrophotometry, activity  of Lactate Dehydrogenase (LDH) and Creatinine Kinase (CPK) were analysed in the plasma by standard colorimetric technique. The lungs of the animal were also excised for histological studies. The results obtained revealed an increase in the mean activity of LDH and CPK in the test groups when compared with the control. There is significant increase in the mean concentrations of LDH and CPK between Group II and controls. (P ≤ 0.05), but the increase is not significant between group I and controls. Also, the histological pictures of the test animals revealed evidence of lungs pathology. This results suggesting that cigarette smoking has associated health hazards, and thus added to the avalanche of overwhelming evidences against cigarette smoking. However this is not clearly manifested in the activities of the enzymes associated with cardiac functions.

Key words: Cigarette, Cardiac enzymes, Smokers and Cadmium.

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