In Silico evaluation of bromelain from stem and fruit of pineapple (Ananas comosus)

Oluwasegun Victor Omotoyinbo, Ini-Obong Emmanuel Ekpenyong, Toluwase Hezekiah Fatoki, Morakinyo David Sanni

 

Full Length Research Paper  I Published March,2018

 

Journal of Agricultural Science and Food Technology  Vol. 4 (2), pp.32-40

 

ABSTRACT

Pineapple (Ananas comosus) is the leading edible member of the family Bromeliaceae, and has been used as a medicinal plant in several native cultures. The medicinal qualities of pineapple are attributed to the enzyme Bromelain which is abundant in the stem and fruit of the pineapple plant and can also be isolated in small amount from pineapple waste such as core, leaves, peel etc. In this study, in silico analysis of bromelain from pineapple stem and fruit was done using standard bioinformatics tools such as Blastp, HMMER, Clustal O, OMA Browser, EMBOSS, Swiss model, and Hex. The result showed specific protein from Gossypium raimondii, Amborella trichopoda, Populus trichocarpa, Corchorus olitorius, Bromelia fastuosa as homologs of bromelain present in pineapple. The theoretical isoelectric point of stem bromelain was 8.436, while that of fruit bromelain was 4.726. The phylogenetic analysis shows that fruit bromelain evolved slowly compared to stem bromelain. Heuristic search for distant homologs implicated Asian wild rice. Eudicots and rodent were among orthologs obtained. The modeled structure obtained revealed that both stem and fruit bromelain possessed secondary structures made up of mainly helixes, coils and beta strands.

Key Words: Bromelain, In silico, physicochemical properties, phylogeny, structure.

 

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