Monitoring Proteolysis in Milk Caused by Pseudomonas fluorescens by the TNBS method

Chove L.M., Grandison A. and Lewis M.


Full Length Research Paper  I Published November,2017


Journal of Agricultural Science and Food Technology  Vol. 3 (6), pp.110-116





The objective of this study was to monitor proteolysis of milk during storage at 20 and 4° C by two strains of Pseudomonas fluorescens namely: NCIMB 702085 (416) and NCIMB 701274 (414) by the TNBS method. Since commercial Pseudomonas fluorescens enzymes are not available, live Pseudomonas fluorescens microorganisms were inoculated into milk followed by extraction and purification by dialysis. Bacterial enzymes obtained were then inoculated into UHT skim milk. Non-inoculated milk was used as a control. Results obtained showed that Pseudomonas fluorescens NCIMB 702085 was more proteolytic than Pseudomonas fluorescens NCIMB 701274. It was concluded that the TNBS method proved useful in monitoring proteolysis in milk by Pseudomonas fluorescens.

Key Words:
Bacterial enzymes, dialysis, purification, UHT skim milk.

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